NAD+

Increased energy is commonly reported, especially with injectable routes. Some people notice improved sleep quality and mental sharpness. Effects can be subtle or pronounced depending on your baseline NAD+ status. Those with significantly depleted levels tend to notice more dramatic improvements.

Sustained energy improvements. Better exercise recovery is often noted. Cognitive benefits may become more noticeable as cellular repair processes ramp up.

The cellular level benefits (DNA repair, mitochondrial support, sirtuin activation) accumulate over time but are not directly perceptible. Continued use maintains elevated NAD+ levels and their associated benefits.

NAD+ levels gradually return to baseline. There is no withdrawal or rebound effect, but benefits will diminish over weeks to months without continued supplementation.

If your mitochondria are damaged, just adding more NAD+ might not work. Converting NAD+ precursors into usable NAD+ requires ATP. If your mitochondria cannot produce ATP efficiently, they cannot use NAD+ precursors effectively. This is exactly why some people supplement with NAD+ and do not feel anything. The machinery is too broken to use the fuel. This is where SS-31 comes in first for the repair phase.

For research only

LOADING PROTOCOL

Dose:           100 to 200 mg daily

Duration:       7 to 10 consecutive days

Purpose:        Rapidly restore depleted cellular reserves

MAINTENANCE PROTOCOL

Dose:           50 to 100 mg

Frequency:      1 to 3 times per week

Timing:         Morning preferred (may interfere with sleep if taken late)

Do 8 to 12 weeks on NAD+ supplementation, then 4 to 8 weeks off. This prevents NAMPT suppression and gives your body's natural production pathways a chance to recover. Exercise during the off-cycle helps restore natural NAD+ production.

NAD+ requires more bacteriostatic water than most peptides. It is a large molecule that can crystallize or precipitate out of solution when the concentration is too high. The standard 2 mL reconstitution used for most peptides does not work here.

RECONSTITUTION

500 mg vial:    Add 3 mL bacteriostatic water

               Concentration: 166.7 mg/mL

               For 100 mg dose, draw 60 units on insulin syringe

After reconstitution. Use within 14 days (not the typical 28 days for most peptides). Do not freeze. Protect from light. Before every injection, inspect the solution. It should be clear and colorless. If you see any cloudiness, discoloration, or particles, the NAD+ has precipitated out of solution and that vial should be discarded.